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Material and method: Sixty patients were included in our study. All of the patient underwent colposcopy-guided biopsy to asses the grade of CIN. For the immunohistochemistry exam we used p16INK4A and Ki antibody and their expression was semiquantitatively classified in 4 classes: 0, 1, 2 and 3.

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Background 1. Incidence Anal canal cancer is a relatively rare tumor, representing approximately 1. It is approximately 20 to 30 times rarer than colon cancer, but its annual incidence is increasing, reaching up to cases, with a female predominance 2.

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Atypical squamous cells cannot exclude high grade squamous intraepithelial lesion ASC-Ha recognized category in the Bethesda Nomenclature System for cervical cytology. Limited follow-up data are available on patients with this cervical cytology results. ASC-H is a large umbrella of citologic anomalies that can hide a severe cervical lesion or an atrofic or an inflammatory modification with no special signification.