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Cu aproximativ 12 ore nainte de ovulaie, dimensiunea foliculului descrete cu mm, acesta devine mai fluctuent, iar forma sa rotund se transform ntr-una triunghiular neregulat, datorit descreterii presiunii interioare. Stabilirea momentului ovulaiei se face prin examene transrectale repetate, precum i prin ecografie. Se urmrete efectuarea inseminrii ct mai aproape de momentul ovulaiei, astfel nct s rezulte un indice de inseminare optim. Vacile trebuie s aib cel puin dou luni de la parturiie.

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In case of dead animals, the specimens shluld be taken soon after seath to avoid the chances of invasion of tissues by putrefactive bacteria rendering the specimens unsuitable for testicular cancer xenograft. Generous blocks of fresh tissues such as liver, spleen, kidneys, lymph nodes, lungs and brain may be forwarded refrigerated but not frozen in wide mouth sterile bottles, when the examination is to be carried out within a short period after collection. While forwarding material in this preservative, it would be better to send larger pieces of the tissues, so that if necessary, cultures may be attempted from their central areas where the glycerine penetration has been the least.

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